{"product_id":"pkm1-71-2-5-monoclonal-antibody-bs66502","title":"PKM1 (71-2-5) Monoclonal Antibody - BS66502","description":"\u003cp\u003ePKM1 (71-2-5) Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS66502-50, BS66502-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Pyruvate kinase is a glycolytic enzyme that catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adμlt tissues. The M2 isoform (PKM2) is an alternatively spliced variant of M1 that is expressed during embryonic development. Research studies found that cancer cells exclusively express PKM2. PKM2 is shown to be essential for aerobic glycolysis in tumors, known as the Warburg effect. When cancer cells switch from the M2 isoform to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased. These cells also show decreased tumorigenicity in mouse xenografts. Recent studies showed that PKM2 is not essential for all tumor cells. In the tumor model studied, PKM2 was found to be active in the non-proliferative tumor cell popμlation and inactive in the proliferative tumor cell popμlation.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat, Mouse\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: IF, IHC\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, Opa-interacting protein 3, Pyruvate kinase 2\/3, Pyruvate kinase muscle isozyme, Threonine-protein kinase PKM2, PKM, OIP3, PK2, PK3, PKM2\u003c\/p\u003e\n\n\u003cp\u003eClonality: Monoclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein of Human PKM1.\u003c\/p\u003e\n\n\u003cp\u003eDilution: IF: 1:50~1:200\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from cell cμlture supernatant by protein A+G and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: PKM1(71-2-5) polyclonal antibody detects endogenous levels of PKM1 protein.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 65 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: P14618\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG, 0.5mg\/ml in PBS with 0.05% Proclin300, 50% glycerol, 0.2% BSA, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45938584322227,"sku":"BS66502-50","price":289.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45938584354995,"sku":"BS66502-100","price":642.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_7d54f7f7-895a-4faa-805b-93c7ca9b170c.jpg?v=1788296274","url":"https:\/\/afsbio.com\/products\/pkm1-71-2-5-monoclonal-antibody-bs66502","provider":"AFSBio Inc.","version":"1.0","type":"link"}