{"product_id":"pkn1-polyclonal-antibody-bs5859","title":"PKN1 Polyclonal Antibody-BS5859","description":"\u003cp\u003ePKN1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS5859-50, BS5859-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Serine\/threonine-protein kinase N1; Protease-activated kinase 1; PAK-1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Serine-threonine protein kinase N; PAK1; PKN; PRK1; PRKCL1\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q16512\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nICC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Protein kinase N (PKN) is a fatty acid-activated serine\/threonine kinase whose catalytic domain exhibits homology with that of the PKC family. PKN associates with Rho via its amino terminus, is activated in a GTP-dependent manner and phosphorylates the head-rod domain of neurofilament protein. A second protein, rhophilin, exhibits 40% sequence identity with the amino terminal Rho binding domain. The enzymatic activity of rhophilin has not been demonstrated and it is possible that it acts through the recruitment of cytoskeletal components that initiate a kinase signaling cascade. Citron interacts specifically with active Rho and Rac1 but not Cdc42. Citron exhibits a distinctive protein organization and little homology with the Rho binding domains of PKN and rhophilin.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: PKN1 polyclonal antibody detects endogenous levels of PKN1 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 103, 120 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of PKN1 polyclonal antibody at 1:500 dillution\u003cbr\u003e\nLane 1: MCF-7 whole cell lysate(40μg)\u003cbr\u003e\nLane 2: AML-12 whole cell lysate(40μg)\u003cbr\u003e\nLane 3: BV2 whole cell lysate(40μg)\u003cbr\u003e\nLane 4: C6 whole cell lysate(40μg) \u003cbr\u003e\nLane 5: H9C2 whole cell lysate(40μg)\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 294-339 of Human PKN1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42911820251315,"sku":"BS5859-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42911820284083,"sku":"BS5859-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_d6aeec10-7686-464b-885a-c1693d76c249.jpg?v=1703180011","url":"https:\/\/afsbio.com\/products\/pkn1-polyclonal-antibody-bs5859","provider":"AFSBio Inc.","version":"1.0","type":"link"}