{"product_id":"pml-p39-polyclonal-antibody-bs1296","title":"PML (P39) Polyclonal Antibody-BS1296","description":"\u003cp\u003ePML (P39) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1296-50, BS1296-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P29590\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eBackground: The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t (15;17) (q22;q11.2-q12) chromosomal trans-location of the retinoic acid receptor α (RARα) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML\/RARα chimeric protein. Myeloid precursor cells expressing the PML\/ RARα chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML\/RARα transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML\/ RARα also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: PML (P39) polyclonal antibody detects endogenous levels of PML protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of PML (P39) pAb at 1:500 dilution\u003cbr\u003e\nLane 1: L02 whole cell lysate (40ug)\u003cbr\u003e\nLane 2: HEK293T whole cell lysate (40ug)\u003cbr\u003e\nLane 3: A549 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of PML (P39) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 98 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Protein PML; Promyelocytic leukemia protein; RING finger protein 71; Tripartite motif-containing protein 19; MYL; PP8675; RNF71; TRIM19\u003c\/p\u003e\n\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to the N-terminal of Human PML.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953208692915,"sku":"BS1296-50","price":254.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953208725683,"sku":"BS1296-100","price":442.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_80305b1d-8a7b-46ad-ae2c-cc99dfb0cece.jpg?v=1705056799","url":"https:\/\/afsbio.com\/products\/pml-p39-polyclonal-antibody-bs1296","provider":"AFSBio Inc.","version":"1.0","type":"link"}