{"product_id":"pnpt1-polyclonal-antibody-bs62427","title":"PNPT1 Polyclonal Antibody-BS62427","description":"\u003cp\u003ePNPT1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS62427-50, BS62427-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q8TCS8\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB  IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500~1:1000  IHC:1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Mitochondrial polyribonucleotide nucleotidyltransferase, also designated 3'-5' RNA exonuclease, OLD35, PNPase or PNPT1, is an evolutionaly conserved protein in which the mouse protein shares 90% identity with the human version. PNPase participates in mRNA degradation and hydrolyzes single-stranded ribonucleotides in the 3' to 5' direction. PNPase forms homotrimers and is upregulated in response to interferon-β induction. The N-terminus of PNPase contains a putative mitochondrial targeting sequence; mutation analysis confirms that N-terminal sequences of PNPase target the protein to the mitochondria. Endogenous PNPase also co-localizes with a mitochondrial marker protein in HeLa cells.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The protein was purified from E.coli and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -24°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: PNPT1 polyclonal antibody detects endogenous levels of PNPT1 protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of PNPT1 polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLaneA:PC12 whole cell lysate\u003cbr\u003e\nLaneB:MEF whole cell lysate\u003cbr\u003e\nLaneC:A549 whole cell lysate\u003cbr\u003e\nLaneD:L02 whole cell lysate\u003cbr\u003e\nLaneE:HCT116 whole cell lysate\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 88 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Polyribonucleotide nucleotidyltransferase 1, mitochondrial; 3'-5' RNA exonuclease OLD35; PNPase old-35; Polynucleotide phosphorylase 1; PNPase 1; Polynucleotide phosphorylase-like protein; PNPT1; PNPASE\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human PNPT1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910457561267,"sku":"BS62427-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910457594035,"sku":"BS62427-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_11df0055-ec0e-4bbc-9022-ec758b108573.jpg?v=1703092788","url":"https:\/\/afsbio.com\/products\/pnpt1-polyclonal-antibody-bs62427","provider":"AFSBio Inc.","version":"1.0","type":"link"}