{"product_id":"porcine-cortisol-elisa-kit-e-el-0159","title":"Porcine Cortisol ELISA Kit - E-EL-0159","description":"\u003cp\u003ePorcine Cortisol ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 24T, 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-EL-0159-24T, E-EL-0159-48T, E-EL-0159-96T\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Cortisol\u003c\/p\u003e\n\n \n\n \n\n\u003cp\u003eResearch Areas: Signal transduction, Metabolism\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Porcine\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eUsage: This ELISA kit applies to the in vitro quantitative determination of Porcine Cortisol concentrations in serum, plasma.\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Serum, Plasma\u003c\/p\u003e\n\n\u003cp\u003eType: Competitive\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 2.5h\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colormetric\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.48 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.78-50 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Porcine Cortisol. During the reaction, Porcine Cortisol in the sample or standard competes with a fixed amount of Porcine Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Porcine Cortisol. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Porcine Cortisol in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eCross Reactivity: This kit recognizes Porcine Cortisol in samples. No significant cross-reactivity or interference between Porcine Cortisol and analogues was observed\u003c\/p\u003e\n\n\u003cp\u003eRecovery Rate: 80%-120%\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Both intra-CV and inter-CV are \u0026lt; 10%.\u003c\/p\u003e\n\n\u003cp\u003eStorage: 2-8℃\/-20℃, 12 months\u003c\/p\u003e\n\n\u003cp\u003eShipping: ice bag\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"24T","offer_id":43006601494707,"sku":"E-EL-0159-24T","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":43006601527475,"sku":"E-EL-0159-48T","price":554.4,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43006601560243,"sku":"E-EL-0159-96T","price":693.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/Untitled-1_35354b73-e766-497f-a261-04d818803b86.jpg?v=1706531393","url":"https:\/\/afsbio.com\/products\/porcine-cortisol-elisa-kit-e-el-0159","provider":"AFSBio Inc.","version":"1.0","type":"link"}