{"product_id":"pou2af1-polyclonal-antibody-bs8364","title":"POU2AF1 Polyclonal Antibody-BS8364","description":"\u003cp\u003ePOU2AF1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS8364-50, BS8364-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q16633\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Transcriptional coactivator that specifically associates with either POU2F1\/OCT1 or POU2F2\/OCT2. It boosts the POU2F1\/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2\/OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of POU2F1\/OCT1 and POU2F2\/OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Regulates IL6 expression in B cells as POU2F2\/OCT2 coactivator (By similarity.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 27kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western Blot analysis of extracts of various cell lines, using POU2AF1 antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 90s.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: POU2AF1; BOB1; OBF-1; OBF1; OCAB\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human POU2AF1(NP_006226.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42929144332467,"sku":"BS8364-50","price":334.88,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42929144365235,"sku":"BS8364-100","price":576.38,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_d5cdd7df-2831-4fe1-ba23-6d799d5bb98e.jpg?v=1704198322","url":"https:\/\/afsbio.com\/products\/pou2af1-polyclonal-antibody-bs8364","provider":"AFSBio Inc.","version":"1.0","type":"link"}