{"product_id":"ppp4c-monoclonal-antibody-mb0060","title":"PPP4C Monoclonal Antibody-MB0060","description":"\u003cp\u003ePPP4C Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB0060-50, MB0060-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P60510\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC ICC\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:1000\u003cbr\u003e\nICC: 1:50~200\u003cbr\u003e\nIHC: 1:500\u003c\/p\u003e\n\n\u003cp\u003eBackground: Protein phosphatase 4C may be involved in microtubule organization. It binds 1 iron ion and 1 manganese ion per subunit. PP4 consists of a catalytic subunit PPP4C and a regulatory subunit. PPP4R1 and belongs to the PPP phosphatase family, PP X subfamily.Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity)\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of PPP4C and does not cross-react with related proteins\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Predicted band size: 34KDa \u003cbr\u003e\nObserved band size: 34KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot detection of Protein Phosphatase 4C antibody in Hela, 293 and Jurkat cell lysates using Protein Phosphatase 4C antibody (1:1000 diluted)., IHC of paraffin-embedded human breast cancer using anti-Protein Phosphatase 4C diluted 1\/500-1\/1000., Immunocytochemistry of HeLa cells using anti- Protein Phosphatase 4C antibody diluted 1:200.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Serine\/threonine-protein phosphatase 4 catalytic subunit; PP4C; Pp4; Protein phosphatase X; PP-X; PPP4C; PPP4; PPX\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant full length Human PPP4C.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42997588361395,"sku":"MB0060-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42997588394163,"sku":"MB0060-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e93e221f-4896-46f0-8cac-29aad6d2b0a9.jpg?v=1706270787","url":"https:\/\/afsbio.com\/products\/ppp4c-monoclonal-antibody-mb0060","provider":"AFSBio Inc.","version":"1.0","type":"link"}