{"product_id":"prak-phospho-thr182-rabbit-pab-es7817","title":"PRAK (phospho Thr182) rabbit pAb - ES7817","description":"\u003cp\u003ePRAK (phospho Thr182) rabbit pAb\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES7817-50, ES7817-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene is a tumor suppressor and member of the serine\/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1\/ERK, MAPK14\/p38-alpha, and MAPK11\/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012],\u003c\/p\u003e \n\n\u003cp\u003eAlternate Name: MAPKAPK5; PRAK; MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAP-K5; MAPKAPK-5; MK-5; MK5; p38-regulated\/activated protein kinase; PRAK\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB; IHC; IF; ELISA\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. ELISA: 1\/5000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human; Mouse\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human MAPKAPK5 around the phosphorylation site of Thr182. AA range:148-197\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 60kD\u003c\/p\u003e\n\n\u003cp\u003eHuman Gene ID: 8550\u003c\/p\u003e\n\n\u003cp\u003eHuman SWISS Prot NO: Q8IW41\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Cytoplasm. Nucleus. Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3\/MAPK6 or ERK4\/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA\/PRKACA at Ser-115 also induces nuclear export.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"50μL","offer_id":45202359124147,"sku":"ES7817-50","price":207.2,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45202359156915,"sku":"ES7817-100","price":347.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_3a541e80-4f00-411c-883d-f4e804e80eb9.png?v=1767127409","url":"https:\/\/afsbio.com\/products\/prak-phospho-thr182-rabbit-pab-es7817","provider":"AFSBio Inc.","version":"1.0","type":"link"}