{"product_id":"prolyl-hydroxylase-phd1-egln2-polyclonal-antibody-bs9885m","title":"Prolyl hydroxylase PHD1 (EGLN2) Polyclonal Antibody-BS9885M","description":"\u003cp\u003eProlyl hydroxylase PHD1 (EGLN2) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50 µl, 100 µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS9885M-50, BS9885M-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q96KS0\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eProlyl hydroxylase PHD1 (EGLN2) Polyclonal Antibody-BS9885M\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000  |  IP,1:20 - 1:50\u003c\/p\u003e\n\n\u003cp\u003eBackground: The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 43kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using Prolyl hydroxylase PHD1 ) antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 30s., Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug Prolyl hydroxylase PHD1 ) antibody. Western blot was performed from the immunoprecipitate using Prolyl hydroxylase PHD1 ) antibody at a dilution of 1:1000.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: EGLN2; EIT6; HIF-PH1; HIFPH1; HPH-1; HPH-3; PHD1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide of human hydroxylase PHD1 (EGLN2))(NP_444274.1).\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42968447877299,"sku":"BS9885M-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42968447910067,"sku":"BS9885M-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_346a77b3-c6d0-4b47-b36a-36dceb8396d6.jpg?v=1705427995","url":"https:\/\/afsbio.com\/products\/prolyl-hydroxylase-phd1-egln2-polyclonal-antibody-bs9885m","provider":"AFSBio Inc.","version":"1.0","type":"link"}