{"product_id":"prpf19-polyclonal-antibody-bs2689","title":"PRPF19 Polyclonal Antibody-BS2689","description":"\u003cp\u003ePRPF19 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2689-50, BS2689-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Pre-mRNA-processing factor 19; Nuclear matrix protein 200; PRP19\/PSO4 homolog; hPso4; RING-type E3 ubiquitin transferase PRP19; Senescence evasion factor; PRPF19I; NMP200; PRP19; SNEV\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9UMS4\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: The spliceosome, the 0gigantic molecular machine that performs pre-mRNA splicing in eukaryotes, contains over 200 different proteins and five RNA molecules (U1, U2, U4, U5 and U6). Pre-mRNA splicing is essential to remove internal non-coding regions of pre-mRNA (introns) and to join the remaining segments (exons) into mRNA before translation. The PRP19-associated complex is required for stable association of U5 and U6 with the spliceosome after U4 is released. Changes within the spliceosome upon binding of the PRP19-associated complex include remodeling of the U6\/5‘ splice site interaction and destabilization of Lsm proteins to allow further interaction of U6 with the intron sequence.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: PRPF19 polyclonal antibody detects endogenous levels of PRPF19 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 55 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of PRPF19 polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLane 1: The Brain tissue lysate of Rat(20ug)\u003cbr\u003e\nLane 2: The Brain tissue lysate of Mouse(20ug)\u003cbr\u003e\nLane 3: MCF-7 whole cell lysate(10ug)\u003cbr\u003e\nLane 4: Hela whole cell lysate(10ug)\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding Human PRPF19.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42911797575859,"sku":"BS2689-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42911797608627,"sku":"BS2689-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_083aa54d-e2c3-4856-b394-5712431d1d74.jpg?v=1703179382","url":"https:\/\/afsbio.com\/products\/prpf19-polyclonal-antibody-bs2689","provider":"AFSBio Inc.","version":"1.0","type":"link"}