{"product_id":"prpf8-polyclonal-antibody-bs91119","title":"PRPF8 Polyclonal Antibody-BS91119","description":"\u003cp\u003ePRPF8 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS91119-50, BS91119-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q6P2Q9(Human) Q99PV0(Mouse)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC IHC FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500-1:2,000\u003cbr\u003e\nICC:1:50-1:200\u003cbr\u003e\nIHC:1:50-1:200\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: PRP8, also designated pre-mRNA-processing-splicing factor 8, is a highly conserved nuclear protein and a central component of the catalytic core of the spliceosome, where it may be involved in various molecular rearrangements. PRP8, which is widely expressed, plays a role in transesterification reactions that regulate splicesome-induced pre-mRNA splicing. Specifically, PRP8 interacts with the GU dinucleotide at the 5' splice site (5'SS) and forms a specific UV-inducible cross-link. It also interacts functionally with the 3'SS, affecting the efficiency of the second catalytic step. PRP8 may play a role in the first transesterification step, as PRP8 mutations that prohibit negative regulation of PRP28 or PRP44\/Brr2 subsequently block U4 activation. In addition, PRP8 interacts with a conserved region of U6 that is instrumental in the formation of the catalytic core of the spliceosome.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: PRPF8 polyclonal antibody detects endogenous levels of PRPF8 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 274 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of PRPF8 on SiHa (1) and A549 (2) cell lysate using anti-PRPF8 antibody at 1\/1,000 dilution., ICC staining PRPF8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: PRPF8, PRPC8, 220 kDa U5 snRNP specific protein, 220 kDa U5 snRNP-specific protein, Apoptosis regulated protein 1, Apoptosis regulated protein 2, HPRP8, p220, Pre mRNA processing factor 8, Pre mRNA-processing factor 8, S. cerevisiae, homolog of, Pre-mRNA-processing-splicing factor 8, Precursor mRNA processing protein, PRP8, PRP8 homolog, PRP8 pre mRNA processing factor 8 homolog, PRP8, PRPC8, Prpf8, Retinitis pigmentosa 13 (autosomal dominant), RP13, SNRNP220, Splicing factor Prp8, U5 snRNP specific protein, U5 snRNP specific protein (220 kD), ortholog of S. cerevisiae Prp8p,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Recombinant protein within 1–200 of human PRPF8.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42984616427699,"sku":"BS91119-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42984616460467,"sku":"BS91119-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_d3a021f4-943a-4d8c-b679-6658876056e9.jpg?v=1705919416","url":"https:\/\/afsbio.com\/products\/prpf8-polyclonal-antibody-bs91119","provider":"AFSBio Inc.","version":"1.0","type":"link"}