{"product_id":"pu-1-spi1-polyclonal-antibody-bs91136","title":"PU.1\/Spi1 Polyclonal Antibody-BS91136","description":"\u003cp\u003ePU.1\/Spi1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS91136-50, BS91136-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P17947(Human)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500-1:1,000\u003cbr\u003e\nIHC:1:50-1:200\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: The Ets transcription factor family (Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF) are DNA-binding proteins that influence lymphoid development and activity. The Ets family monomeric proteins bind the consensus DNA site GGA(A\/T) through a unique winged helix-turn-helix motif known as the Ets domain. PU.1 (Spi-1\/Spi-A), Spi-B and Spi-C are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to the non-canonical AGAA sites. PU.1 transactivates a large number of B cell genes, such as those encoding CD72, CD20 and Btk, and Spi-B enhances expression of many of these same target genes. PU.1 is expressed in a wide variety of hematopoetic cells, including B cells, early T-cells, megakaryocytes, granulocytes, mast cells, immature erythrocytes and myeloid cells. Alternatively, Spi-B expression is limited to B cells and immature T cells, where expression accumulates through T-lineage commitment and then is dramatically absent following the beta-selection checkpoint.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: PU.1\/Spi1 polyclonal antibody detects endogenous levels of PU.1\/Spi1 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 31 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of PU.1\/Spi1 on THP-1 cell using anti-PU.1\/Spi1 antibody at 1\/500 dilution., Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PU.1\/Spi1 antibody. Counter stained with hematoxylin.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: SPI1, transcription factor spi1, 31 kDa Transforming Protein, 31 kDa-transforming protein, cb1086, Hematopoietic transcription factor PU.1, OF, oncogene spi1, PU.1, SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of, SFPI1, si:by184l24.2, SPI 1, SPI 1 proto oncogene, SPI A, Spi1, SPI1, Spleen focus forming virus (SFFV) proviral integration oncogene spi1, Spleen focus forming virus proviral integration oncogene spi1, Transcription factor PU.1,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42984617509043,"sku":"BS91136-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42984617541811,"sku":"BS91136-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_2d5ffcac-1673-45af-b6d9-1a392b1811eb.jpg?v=1705919442","url":"https:\/\/afsbio.com\/products\/pu-1-spi1-polyclonal-antibody-bs91136","provider":"AFSBio Inc.","version":"1.0","type":"link"}