{"product_id":"puma-r169-polyclonal-antibody-bs2922","title":"PUMA (R169) Polyclonal Antibody - BS2922","description":"\u003cp\u003ePUMA (R169) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2922-50, BS2922-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: PUMA (Bcl-2 binding component 3, JFY1, PUMA\/JFY1) is a mitochondrial pro-apoptotic Bcl-2 homology domain (BH3)-only protein that induces rapid apoptosis through a Bax- and mitochondria-dependent pathway. The PUMA gene encodes four proteins originating from different splice variants of the same transcript: PUMAα, β, γ and δ. Both PUMAα and PUMAβ contain a BH3 domain, while PUMAγ and PUMAδ lack this domain. The BH3 domain is essential for binding of PUMAα and PUMAβ to Bcl-2 or Bcl-xL. PUMA is an initiator of γ-radiation apoptosis and glucocorticoid-induced apoptosis in lymphoid cells in vivo. Bcl-2 family members generally regμlate apoptosis and transmit death signals to mitochondria. Members of this family include both pro- and anti-apoptotic proteins that share homologous sequences known as Bcl-2 homology domains (BH1-4). The BH3 proteins, BID, NOXA, PUMA, NBK, Bim and Bad, are all pro-apoptotic and share sequence homology within the amphipathic α-helical BH3 region.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Bcl-2-binding component 3, JFY-1, p53 up-regμlated modμlator of apoptosis, BBC3, PUMA\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 132-183 of Human PUMA.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: PUMA (R169) polyclonal antibody detects endogenous levels of PUMA protein.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 15, 20, 28 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: Regμlation of Apoptosis, Mitochondrial Control of Apoptosis,\u003c\/p\u003e \n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: Q9BXH1\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45946247970995,"sku":"BS2922-50","price":274.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946248003763,"sku":"BS2922-100","price":457.5,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/puma-r169-polyclonal-antibody-bs2922","provider":"AFSBio Inc.","version":"1.0","type":"link"}