{"product_id":"puma-rabbit-monoclonal-antibody-bs9849m","title":"PUMA Rabbit Monoclonal Antibody-BS9849M","description":"\u003cp\u003ePUMA Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS9849M-50, BS9849M-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9BXH1\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF, IHC, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000-1:2000 \u003cbr\u003e\nICC\/IF: 1:50-1:200\u003cbr\u003e\nIHC: 1:200-1:500 \u003cbr\u003e\nFC: 1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: PUMA (Bcl-2 binding component 3, JFY1, PUMA\/JFY1) is a mitochondrial pro-apoptotic Bcl-2 homology domain (BH3)-only protein that induces rapid apoptosis through a Bax- and mitochondria-dependent pathway. The PUMA gene encodes four proteins originating from different splice variants of the same transcript: PUMAα, β, γ and δ. Both PUMAα and PUMAβ contain a BH3 domain, while PUMAγ and PUMAδ lack this domain. The BH3 domain is essential for binding of PUMAα and PUMAβ to Bcl-2 or Bcl-xL. PUMA is an initiator of γ-radiation apoptosis and glucocorticoid-induced apoptosis in lymphoid cells in vivo. Bcl-2 family members generally regulate apoptosis and transmit death signals to mitochondria. Members of this family include both pro- and anti-apoptotic proteins that share homologous sequences known as Bcl-2 homology domains (BH1-4). The BH3 proteins, BID, NOXA, PUMA, NBK, Bim and Bad, are all pro-apoptotic and share sequence homology within the amphipathic α-helical BH3 region.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of PUMA and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 20 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of PUMA Rabbit mAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: Hela whole cell lysate\u003cbr\u003e\n Lane 2: K562 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin., ICC staining PUMA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Bcl-2-binding component 3; JFY-1; p53 up-regulated modulator of apoptosis; BBC3; PUMA\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant antibody.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43004764782771,"sku":"BS9849M-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43004764815539,"sku":"BS9849M-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_ffc66e71-d162-4ae4-af44-f2b49d1f2b54.jpg?v=1706445985","url":"https:\/\/afsbio.com\/products\/puma-rabbit-monoclonal-antibody-bs9849m","provider":"AFSBio Inc.","version":"1.0","type":"link"}