{"product_id":"rabphilin-3a-p231-polyclonal-antibody-bs1590","title":"Rabphilin-3A (P231) Polyclonal Antibody-BS1590","description":"\u003cp\u003eRabphilin-3A (P231) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1590-50, BS1590-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9Y2J0\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eBackground: The carboxy-terminal of rabphilin-3A consists of two C2 domains, A and B,and the amino-terminal (residues 45-170) contains a cysteine-rich rich region with two zinc finger motifs. Rabphilin-3A belongs to a family of other carboxyterminal type (C-type) tandem C2 proteins, which includes synaptotagmins and Doc2. Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. Rabphilin-3A binds Rab3a\/GTP\/Mg+ within amino-terminal residues 45 and 170. Rabphilin-3A binds calcium ions and phosphatidylinositol 4,5-bisphophate containing lipid vesicles within its C2 domains. Rabphilin-3A is a positive regulator of calcium dependent exocytosis, while Rab3a is a negative regulator of exocytosis. Although rabphilin-3A associates with Rab3a, they seem to influence exocytosis independently of each other. Rabphilin-3A effects are likely mediated through interactions with an unknown factor that recognizes the Rab3 binding domain.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Rabphilin 3A (P231) polyclonal antibody detects endogenous levels of Rabphilin 3A protein\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Rabphilin-3A (P231) polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLane 1: THP-1 whole cell lysate\u003cbr\u003e\nLane 2: NIH-3T3 whole cell lysate\u003cbr\u003e\nLane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of Rabphilin-3A (P231) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 75 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Rabphilin-3A; Exophilin-1; Exophilin 1; Exophilin1; Rph3a\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 200-250 of Human Rabphilin-3A.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953212919987,"sku":"BS1590-50","price":254.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953212952755,"sku":"BS1590-100","price":442.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_9e9a9779-4a90-4ad3-80a2-84821fabc64b.jpg?v=1705056874","url":"https:\/\/afsbio.com\/products\/rabphilin-3a-p231-polyclonal-antibody-bs1590","provider":"AFSBio Inc.","version":"1.0","type":"link"}