{"product_id":"rad17-polyclonal-antibody-bt-ap07601","title":"Rad17 Polyclonal Antibody - BT-AP07601","description":"\u003cp\u003eRad17 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μL, 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BT-AP07601-20, BT-AP07601-50, BT-AP07601-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: RAD17 checkpoint clamp loader component encoded by RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. RAD17 checkpoint clamp loader component shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. RAD17 checkpoint clamp loader component binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. RAD17 checkpoint clamp loader component recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of RAD17 checkpoint clamp loader component is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of RAD17, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Epigenetics and Nuclear Signaling\u003c\/p\u003e\n\n\u003cp\u003eTarget Protein: RAD17\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC-p, IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eFull Product Name: Rad17 Antibody\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189\u003c\/p\u003e\n\n\u003cp\u003eStorage: -20°C for 1 year\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight (Da): 77055\u003c\/p\u003e\n\n\u003cp\u003eUniProt Accession: Human: O75943; Mouse: Q6NXW6\u003c\/p\u003e\n\n\u003cp\u003eSynonyms: RAD17; R24L; Cell cycle checkpoint protein RAD17; hRad17; RF-C\/activator 1 homolog\u003c\/p\u003e\n\n\u003cp\u003eGeneID: Human: 5884; Mouse: 19356\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"BT Lab","offers":[{"title":"20μL","offer_id":45239867211955,"sku":"BT-AP07601-20","price":151.2,"currency_code":"CAD","in_stock":true},{"title":"50μL","offer_id":45239867244723,"sku":"BT-AP07601-50","price":252.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45239867277491,"sku":"BT-AP07601-100","price":385.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/logo_6871fa70-e115-4c51-8915-60c28893145e.png?v=1769400170","url":"https:\/\/afsbio.com\/products\/rad17-polyclonal-antibody-bt-ap07601","provider":"AFSBio Inc.","version":"1.0","type":"link"}