{"product_id":"rad21-polyclonal-antibody-bs91145","title":"Rad21 Polyclonal Antibody-BS91145","description":"\u003cp\u003eRad21 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS91145-50, BS91145-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O60216 Human;Q61550 Mouse;Entrez Gene: 314949 Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500-1:2,000\u003cbr\u003e\nICC:1:50-1:200\u003cbr\u003e\nIHC:1:100-1:400\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase\/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3\/CASP3 or caspase-7\/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein affinity purified.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Rad21 polyclonal antibody detects endogenous levels of Rad21 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 120 kDa, predicted band size 72 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Rad21 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.\u003cbr\u003e\n Positive control: \u003cbr\u003e\n Lane 1: Mouse ovary tissue lysate \u003cbr\u003e\n Lane 2: Daudi cell lysate, ICC staining Rad21 in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Rad21 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc and trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1\/100 dilution. The nuclear counter stain is DAPI (blue).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: hHR21, NXP-1, RAD21, HR21, KIAA0078, NXP1, SCC1, CDLS4, Double-strand-break repair protein rad21 homolog, hHR21, HR21, HRAD21, KIAA0078, MCD1, Nuclear matrix protein 1, NXP-1, NXP1, Protein involved in DNA double-strand break repair, RAD21, RAD21 homolog (S. pombe), RAD21 homolog, RAD21, Scc1, SCC1 homolog antibody\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein within human Rad21 aa 250-440.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42984618459315,"sku":"BS91145-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42984618492083,"sku":"BS91145-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e1c73df9-385f-403b-9fcb-5ff415fb66fc.jpg?v=1705919459","url":"https:\/\/afsbio.com\/products\/rad21-polyclonal-antibody-bs91145","provider":"AFSBio Inc.","version":"1.0","type":"link"}