{"product_id":"rad51b-g226-polyclonal-antibody-bs2542","title":"Rad51B (G226) Polyclonal Antibody-BS2542","description":"\u003cp\u003eRad51B (G226) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2542-50, BS2542-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O15315\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The DNA repair proteins Rad50, Rad51A-D, Rad52 and Rad54 A and B are key components of the double-strand break repair pathway (DSBR). The process of homologous recombination is a major DNA repair pathway that operates on DNA double-strand breaks to promote error-free repair. Human Rad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination. In addition to Rad51 protein, five paralogs have been identified in vertebrates, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3. Rad51B is a widely expressed protein that is involved in the repair of double-stranded DNA breaks that can arise during DNA replication. It can form a dimer with Rad51C and this complex shows single-stranded DNAdependent ATPase activity.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Rad51B (G226) polyclonal antibody detects endogenous levels of Rad51B protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 45 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Immunohistochemistry (IHC) analyzes of Rad51B (G226) pAb in paraffin-embedded human colon carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Rad51B (G226) pAb in paraffin-embedded human lung carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Rad51B (G226) pAb in paraffin-embedded human liver carcinoma tissue at 1:50, showing cytoplasmic and nuclear staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: DNA repair protein RAD51 homolog 2; R51H2; RAD51 homolog B; Rad51B; RAD51-like protein 1; RAD51B; RAD51L1; REC2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 201-250 of Human Rad51B.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044050108595,"sku":"BS2542-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044050141363,"sku":"BS2542-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_0a79348c-1d86-49b2-8de8-043dd13bd640.jpg?v=1707739055","url":"https:\/\/afsbio.com\/products\/rad51b-g226-polyclonal-antibody-bs2542","provider":"AFSBio Inc.","version":"1.0","type":"link"}