{"product_id":"rad51c-e185-polyclonal-antibody-bs2421","title":"Rad51C (E185) Polyclonal Antibody-BS2421","description":"\u003cp\u003eRad51C (E185) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2421-50, BS2421-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O43502\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eBackground: The process of homologous recombination is a major DNA repair pathway that operates on DNA double-strand breaks to promote error-free repair. Human Rad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination. In addition to Rad51 protein, five paralogs have been identified in vertebrates, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3. Rad51C binds single- and double-stranded DNA and preferentially binds 3'-end-tailed double-stranded DNA. Rad51C is expressed in a variety of tissues, with highest expression in testis, heart muscle, spleen and prostate.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Rad51C (E185) polyclonal antibody detects endogenous levels of Rad51C protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Rad51C (E185) pAb at 1:500 dilution\u003cbr\u003e\nLane 1: H9C2 whole cell lysate (40ug)\u003cbr\u003e\nLane 2: The Testis tissue lysate of Mouse (40ug)\u003cbr\u003e\nLane 3: MCF-7 whole cell lysate (40ug)\u003cbr\u003e\nLane 4: Hela whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Rad51C (E185) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 50 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: DNA repair protein RAD51 homolog 3; R51H3; RAD51 homolog C; RAD51-like protein 2; RAD51C; RAD51L2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 161-210 of Human Rad51C.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953225437363,"sku":"BS2421-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953225470131,"sku":"BS2421-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_371289cd-6ef2-4e93-81a4-7d4452836b76.jpg?v=1705057146","url":"https:\/\/afsbio.com\/products\/rad51c-e185-polyclonal-antibody-bs2421","provider":"AFSBio Inc.","version":"1.0","type":"link"}