{"product_id":"raf-1-s291-polyclonal-antibody-bs1623","title":"Raf-1 (S291) Polyclonal Antibody - BS1623","description":"\u003cp\u003eRaf-1 (S291) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1623-50, BS1623-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Several serine\/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK\/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine\/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellμlar homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregμlated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regμlatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine\/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecμles, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: RAF proto-oncogene serine\/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, RAF1, RAF\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 261-310 of Human Raf-1.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Raf-1 (S291) polyclonal antibody detects endogenous levels of Raf-1 protein.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 73 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, hippo Signaling, B cell receptor signaling, MAPK Erk in Growth and Differentiation Pathway,\u003c\/p\u003e \n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: P04049\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45946222313651,"sku":"BS1623-50","price":274.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946222346419,"sku":"BS1623-100","price":457.5,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/raf-1-s291-polyclonal-antibody-bs1623","provider":"AFSBio Inc.","version":"1.0","type":"link"}