{"product_id":"raf-b-f594-polyclonal-antibody-ap0422","title":"Raf-B (F594) Polyclonal Antibody-AP0422","description":"\u003cp\u003eRaf-B (F594) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: AP0422-50, AP0422-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P15056\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000~1:2000\u003cbr\u003e\nIF  1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites, including Ser338, Tyr341, Thr491, Ser494, Ser497, and Ser499. p21-activated kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity. Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf. Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively. While A-Raf, B-Raf, and c-Raf are similar in sequence and function, differential regulation has been observed. Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428, and Thr439) and lacks a site equivalent to Tyr341 of c-Raf. Research studies have shown that the B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma. Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301, and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Raf-B (F594) polyclonal antibody detects endogenous levels of Raf-B protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 84 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Raf-B (F594) polyclonal antibody at 1:1000 dilution\u003cbr\u003e\n Lane 1: K562 cell membrane lysate (24ug)\u003cbr\u003e\n Lane 2: The Lung tissue lysate of Mouse (30ug)\u003cbr\u003e\n Lane 3: BV2 whole cell lysate (30ug)\u003cbr\u003e\n Lane 4: PC12 whole cell lysate (30ug), Immunofluorescence analysis of A549 cells using Raf-B (F594) pAb at dilution of 1:200.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Serine\/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF; BRAF1; RAFB1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human Raf-B.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989584023731,"sku":"AP0422-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989584056499,"sku":"AP0422-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_2996a1cc-d778-4eab-997b-e22d9e79f5bd.jpg?v=1706095765","url":"https:\/\/afsbio.com\/products\/raf-b-f594-polyclonal-antibody-ap0422","provider":"AFSBio Inc.","version":"1.0","type":"link"}