{"product_id":"raf1-phospho-s43-polyclonal-antibody-bs94091","title":"Raf1 (Phospho-S43) Polyclonal Antibody-BS94091","description":"\u003cp\u003eRaf1 (Phospho-S43) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS94091-50, BS94091-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P04049(Human) Q99N57(Mouse) P11345(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:1,000-1:2,000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Several serine\/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK\/MAP kinases, ribosomal S6 kinase (Rsk)and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine\/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may play the role of the messenger from membrane tyrosine kinases to Raf-1.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Phospho-Raf1 (S43) on differentLysates using anti-Phospho-Raf1 (S43) antibody at 1\/1,000 dilution. Positive control:\u003cbr\u003e\n Lane 1: NIH\/3T3\u003cbr\u003e\n Lane 2: 293T\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 73 kDa\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Raf1 (Phospho-S43) polyclonal antibody detects endogenous levels of Raf1 protein only when phosphorylated at S43.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: cRaf, RAF1, c Raf, C-raf, C-Raf proto-oncogene, serine\/threonine kinase, CMD1NN, Craf 1 transforming gene, cRaf, Craf1 transforming gene, EC 2.7.11.1, kinase Raf1, Murine sarcoma 3611 oncogene 1, NS5, Oncogene MIL, Oncogene RAF1, OTTHUMP00000160218, OTTHUMP00000207813, OTTHUMP00000209389, Protein kinase raf 1, Proto-oncogene c-RAF, Raf 1, Raf 1 proto oncogene serine\/threonine kinase, RAF, Raf proto oncogene serine\/threonine protein kinase, RAF proto-oncogene serine\/threonine-protein kinase, RAF-1, RAF1, RAF1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV, v raf 1 murine leukemia viral oncogene homolog 1, v-raf murine sarcoma viral oncogene homolog 1, v-raf-1 murine leukemia viral oncogene-like protein 1, vraf1 murine leukemia viral oncogene homolog 1,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser43 of human Raf1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42939210629299,"sku":"BS94091-50","price":415.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42939210662067,"sku":"BS94091-100","price":640.78,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_85a87075-0301-4459-9b3b-b823b45f858a.jpg?v=1704545143","url":"https:\/\/afsbio.com\/products\/raf1-phospho-s43-polyclonal-antibody-bs94091","provider":"AFSBio Inc.","version":"1.0","type":"link"}