{"product_id":"raf1-polyclonal-antibody-bz16471","title":"Raf1 Polyclonal Antibody-BZ16471","description":"\u003cp\u003eRaf1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BZ16471-50, BZ16471-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P04049\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: ICC\/IF, WB, IHC-F, IHC-P, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1\/500-1\/1000  IHC: 1\/50-1\/100  IF: 1\/50-1\/200  ELISA: 1\/10000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine\/threonine specific protein kinases ERK1 and ERK2.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Affinity Purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IgG\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Calculated MW: 73 kDa; Observed MW: 73 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Raf1 in HepG2 lysates using Raf1 antibody., Western blot analysis of Raf1 in rat liver lysates using Raf1 antibody. The Lane on the right is blocked with the synthesized peptide., Immunohistochemistry analysis of paraffin-embedded rat kidney tissue using Raf1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunofluorescence analysis of Raf1 in mouse liver using Raf1 antibody, and DAPI.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: RAF1; RAF; RAF proto-oncogene serine\/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human C-RAF. AA range:251-300\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044067639475,"sku":"BZ16471-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044067672243,"sku":"BZ16471-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_0d181a0c-566b-438c-89ec-9069d6c01b75.jpg?v=1707739454","url":"https:\/\/afsbio.com\/products\/raf1-polyclonal-antibody-bz16471","provider":"AFSBio Inc.","version":"1.0","type":"link"}