{"product_id":"rasgrf1-polyclonal-antibody-bs8617","title":"RASGRF1 Polyclonal Antibody-BS8617","description":"\u003cp\u003eRASGRF1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS8617-50, BS8617-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q13972\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eRASGRF1 Polyclonal Antibody-BS8617\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:2000\u003cbr\u003e\nIHC: 1:50~1:200\u003cbr\u003e\nIF: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: A critical step in signal transduction responses to stimulation of cell surface receptors by their ligands involves the accumulation of Ras proteins in their active GTP-bound state. To reach their active GTP-bound state, Ras proteins must first release bound GDP, a rate limiting step mediated by a guanine nucleotide releasing factor (GRF). The mammalian Ras p21 GRF protein has been designated Ras-GRF1 p140. Ras-GRF1 accelerates release of GDP from H- and N-Ras p21 protein in vitro, but not from the related Ral A or Cdc42Hs GTP-binding proteins.Ras-GRF2 p135 is highly homologous to Ras-GRF1 p140 except in the region between the REM and CDC25 domains and appears to function similarly to Ras-GRF1 p140.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: RASGRF1 polyclonal antibody detects endogenous levels of RASGRF1 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 145kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extract of Mouse brain, using RASGRF1 antibody., Immunofluorescence analysis of U20S cell using RASGRF1 antibody. Blue: DAPI for nuclear staining.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Ras-specific guanine nucleotide-releasing factor 1; Ras-GRF1; Guanine nucleotide-releasing protein; GNRP; Ras-specific nucleotide; exchange factor CDC25; RASGRF1; CDC25; GNRP; GRF1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant full length Human RASGRF1.\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42962824167603,"sku":"BS8617-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42962824200371,"sku":"BS8617-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_a96647de-cf99-4359-98d0-766e1425d23b.jpg?v=1705319157","url":"https:\/\/afsbio.com\/products\/rasgrf1-polyclonal-antibody-bs8617","provider":"AFSBio Inc.","version":"1.0","type":"link"}