{"product_id":"rat-25-oh-d-25-hydroxy-vitamin-d-elisa-kit-elk0843","title":"Rat 25-OH-D (25 Hydroxy Vitamin D) ELISA Kit - ELK0843","description":"\u003cp\u003eRat 25-OH-D (25 Hydroxy Vitamin D) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0843-48, ELK0843-96, ELK0843-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: 25 Hydroxy Vitamin D;\u003c\/p\u003e \n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.93 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 3.13-200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Reproductive science; Genetic science; Nutrition metabolism; Bone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211538399411,"sku":"ELK0843-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211538432179,"sku":"ELK0843-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200644178099,"sku":"ELK0843-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_e77417ff-2c1c-42d7-b559-91d08b4ea357.png?v=1767060811","url":"https:\/\/afsbio.com\/products\/rat-25-oh-d-25-hydroxy-vitamin-d-elisa-kit-elk0843","provider":"AFSBio Inc.","version":"1.0","type":"link"}