{"product_id":"rat-alk-smase-alkaline-sphingomyelinase-elisa-kit-elk2629","title":"Rat Alk-Smase (Alkaline Sphingomyelinase) ELISA Kit - ELK2629","description":"\u003cp\u003eRat Alk-Smase (Alkaline Sphingomyelinase) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK2629-48, ELK2629-96, ELK2629-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase\/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.046 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.16-10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Tissue homogenates and other biological fluids.\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Enzyme and Kinase; Neuroscience;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: Q5EZ72\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213262487731,"sku":"ELK2629-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213262520499,"sku":"ELK2629-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200067002547,"sku":"ELK2629-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_4122ab97-a1b5-42b0-8805-9a9bacbabc43.png?v=1767032238","url":"https:\/\/afsbio.com\/products\/rat-alk-smase-alkaline-sphingomyelinase-elisa-kit-elk2629","provider":"AFSBio Inc.","version":"1.0","type":"link"}