{"product_id":"rat-ca15-3-carbonhydrate-antigen-15-3-elisa-kit-elk11235","title":"Rat CA15-3 (Carbonhydrate Antigen 15-3) ELISA Kit - ELK11235","description":"\u003cp\u003eRat CA15-3 (Carbonhydrate Antigen 15-3) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK11235-48, ELK11235-96, ELK11235-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: CA15-3\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 3.68 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 500 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 7.82-500 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Tumor immunity; Infection immunity;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CA15-3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CA15-3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CA15-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CA15-3 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200702144691,"sku":"ELK11235-48","price":576.8,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200702177459,"sku":"ELK11235-96","price":823.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200702210227,"sku":"ELK11235-5x96","price":3498.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_e9a53b39-f55d-4b20-8ce0-83eef7e41fb6.png?v=1767061860","url":"https:\/\/afsbio.com\/products\/rat-ca15-3-carbonhydrate-antigen-15-3-elisa-kit-elk11235","provider":"AFSBio Inc.","version":"1.0","type":"link"}