{"product_id":"rat-edn2-endothelin-2-elisa-kit-elk6274","title":"Rat EDN2 (Endothelin 2) ELISA Kit - ELK6274","description":"\u003cp\u003eRat EDN2 (Endothelin 2) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK6274-48, ELK6274-96, ELK6274-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: ET2; PPET2; Preproendothelin-2\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 2.66 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 500 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 7.82-500 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cardiovascular biology;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat EDN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EDN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EDN2 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: P23943\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213657211059,"sku":"ELK6274-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213657243827,"sku":"ELK6274-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45201359503539,"sku":"ELK6274-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_ceb3eaf9-89b6-42fb-9dc0-6ab912215eb7.png?v=1767094116","url":"https:\/\/afsbio.com\/products\/rat-edn2-endothelin-2-elisa-kit-elk6274","provider":"AFSBio Inc.","version":"1.0","type":"link"}