{"product_id":"rat-eg-vegf-endocrine-gland-derived-vascular-endothelial-growth-factor-elisa-kit-elk2478","title":"Rat EG-VEGF (Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit - ELK2478","description":"\u003cp\u003eRat EG-VEGF (Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK2478-48, ELK2478-96, ELK2478-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: PK1; EGVEGF; PRK1, PROK1; Prokineticin 1; Black Mamba Toxin-Related Protein; Mambakine\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 6.4 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 1000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 15.63-1000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cytokine; Tumor immunity;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat EG-VEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EG-VEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EG-VEGF in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: Q8R414\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213251969203,"sku":"ELK2478-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213252001971,"sku":"ELK2478-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200061104307,"sku":"ELK2478-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_aa75bda3-4bbd-4cd0-b365-6ea7a37d616c.png?v=1767032062","url":"https:\/\/afsbio.com\/products\/rat-eg-vegf-endocrine-gland-derived-vascular-endothelial-growth-factor-elisa-kit-elk2478","provider":"AFSBio Inc.","version":"1.0","type":"link"}