Catalogue Number: ELK4474
Alternative Names: AXLLG; AXSF; AXL Stimulatory Factor; AXL receptor tyrosine kinase ligand
Species: Rat
Assay Type: Sandwich
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research: Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest Specific Protein 6(GAS6). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Growth Arrest Specific Protein 6(GAS6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest Specific Protein 6(GAS6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Arrest Specific Protein 6(GAS6) in the samples is then determined by comparing the OD of the samples to the standard curve.