{"product_id":"rat-hmgb-1-high-mobility-group-protein-b1-elisa-kit-elk10272","title":"Rat HMGB-1 (High Mobility Group Protein B1) ELISA Kit - ELK10272","description":"\u003cp\u003eRat HMGB-1 (High Mobility Group Protein B1) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK10272-48, ELK10272-96, ELK10272-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMG1; High Mobility Group Protein 1\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.067 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.16-10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Infection immunity;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: P63159\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45199915450547,"sku":"ELK10272-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45199915483315,"sku":"ELK10272-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45199915516083,"sku":"ELK10272-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_071863d5-34eb-40ab-afc4-203f98ea10f4.png?v=1767028907","url":"https:\/\/afsbio.com\/products\/rat-hmgb-1-high-mobility-group-protein-b1-elisa-kit-elk10272","provider":"AFSBio Inc.","version":"1.0","type":"link"}