{"product_id":"rat-il-2rα-cd25-interleukin-2-receptor-alpha-chain-elisa-kit-e-el-r0899","title":"Rat IL-2Rα\/CD25 (Interleukin-2 Receptor alpha chain) ELISA Kit - E-EL-R0899","description":"\u003cp\u003eRat IL-2Rα\/CD25 (Interleukin-2 Receptor alpha chain) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 24T, 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-EL-R0899-24, E-EL-R0899-48, E-EL-R0899-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: IL-2sRα; CD25\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P26897 Research Areas: Cancer, Immunology\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Rat\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eUsage: This ELISA kit applies to the in vitro quantitative determination of Rat IL-2sRα\/CD25 concentrations in serum, plasma and other biological fluids.\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Serum, Plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eType: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colormetric\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 46.88 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 78.13-5000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2sRα\/CD25. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-2sRα\/CD25 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-2sRα\/CD25, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-2sRα\/CD25. You can calculate the concentration of Rat IL-2sRα\/CD25 in the samples by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eCross Reactivity: This kit recognizes Rat IL-2sRα\/CD25 in samples. No significant cross-reactivity or interference between Rat IL-2sRα\/CD25 and analogues was observed\u003c\/p\u003e\n\n\u003cp\u003eRecovery Rate: 80%-120%\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Both intra-CV and inter-CV are \u0026lt; 10%.\u003c\/p\u003e\n\n\u003cp\u003eStorage: 2-8℃\/-20℃, 12 months\u003c\/p\u003e\n\n\u003cp\u003eShipping: ice bag\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"24T","offer_id":43020792561843,"sku":"E-EL-R0899-24","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":43020792594611,"sku":"E-EL-R0899-48","price":681.8,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43020792627379,"sku":"E-EL-R0899-96","price":852.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/Untitled-1_29916204-97ad-4b23-83cb-3987b1c09037.jpg?v=1707046835","url":"https:\/\/afsbio.com\/products\/rat-il-2r%ce%b1-cd25-interleukin-2-receptor-alpha-chain-elisa-kit-e-el-r0899","provider":"AFSBio Inc.","version":"1.0","type":"link"}