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Rat L1CAM (L1-Cell Adhesion Molecule) ELISA Kit

Rat L1CAM (L1-Cell Adhesion Molecule) ELISA Kit

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Catalogue Number: ELK4462

Alternative Names: CD171; L1-CAM; CAML1; HSAS; HSAS1; MASA; MIC5; N-CAML1; S10; SPG1; Antigen Identified By Monoclonal Antibody R1

Species: Rat

Assay Type: Sandwich

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 3.5h

Research: CD & Adhesion molecule;Tumor immunity;Infection immunity;Neuro science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to L1-Cell Adhesion Molecule(L1CAM). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to L1-Cell Adhesion Molecule(L1CAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain L1-Cell Adhesion Molecule(L1CAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of L1-Cell Adhesion Molecule(L1CAM) in the samples is then determined by comparing the OD of the samples to the standard curve.