Catalogue Number: ELK4462
Alternative Names: CD171; L1-CAM; CAML1; HSAS; HSAS1; MASA; MIC5; N-CAML1; S10; SPG1; Antigen Identified By Monoclonal Antibody R1
Species: Rat
Assay Type: Sandwich
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research: CD & Adhesion molecule;Tumor immunity;Infection immunity;Neuro science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to L1-Cell Adhesion Molecule(L1CAM). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to L1-Cell Adhesion Molecule(L1CAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain L1-Cell Adhesion Molecule(L1CAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of L1-Cell Adhesion Molecule(L1CAM) in the samples is then determined by comparing the OD of the samples to the standard curve.