{"product_id":"rat-mep1a-meprin-a-alpha-elisa-kit-elk5520","title":"Rat MEP1a (Meprin A Alpha) ELISA Kit - ELK5520","description":"\u003cp\u003eRat MEP1a (Meprin A Alpha) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK5520-48, ELK5520-96, ELK5520-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 15.4 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 4000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 62.5-4000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Metabolic pathway\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MEP1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MEP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MEP1a in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: Q64230\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213592428723,"sku":"ELK5520-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213592461491,"sku":"ELK5520-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45201342300339,"sku":"ELK5520-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_0f7f0c1b-4370-4420-a4f9-203bce3df273.png?v=1767093345","url":"https:\/\/afsbio.com\/products\/rat-mep1a-meprin-a-alpha-elisa-kit-elk5520","provider":"AFSBio Inc.","version":"1.0","type":"link"}