{"product_id":"rat-nes1-nesfatin-1-elisa-kit-elk4864","title":"Rat NES1 (Nesfatin 1) ELISA Kit - ELK4864","description":"\u003cp\u003eRat NES1 (Nesfatin 1) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK4864-48, ELK4864-96, ELK4864-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: Nesfatin-1\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 183.3 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 40000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 625-40000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Glycolipid metabolism\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NES1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NES1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NES1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211719835827,"sku":"ELK4864-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211719868595,"sku":"ELK4864-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200729735347,"sku":"ELK4864-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_9fe2d863-e302-48b3-9d31-b8e435020521.png?v=1767062764","url":"https:\/\/afsbio.com\/products\/rat-nes1-nesfatin-1-elisa-kit-elk4864","provider":"AFSBio Inc.","version":"1.0","type":"link"}