{"product_id":"rat-nf-κb-p105-nuclear-factor-nf-kappa-b-p105-subunit-microsample-elisa-kit-elk5691ms","title":"Rat NF-κB p105 (Nuclear factor NF-kappa-B p105 subunit) Microsample ELISA Kit - ELK5691MS","description":"\u003cp\u003eRat NF-κB p105 (Nuclear factor NF-kappa-B p105 subunit) Microsample ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK5691MS-48, ELK5691MS-96, ELK5691MS-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.114 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.32-20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: Q63369\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200538894515,"sku":"ELK5691MS-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200538927283,"sku":"ELK5691MS-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200538960051,"sku":"ELK5691MS-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_b6106dce-4e9d-4b16-b3dd-745b8809ef8c.png?v=1767093508","url":"https:\/\/afsbio.com\/products\/rat-nf-%ce%bab-p105-nuclear-factor-nf-kappa-b-p105-subunit-microsample-elisa-kit-elk5691ms","provider":"AFSBio Inc.","version":"1.0","type":"link"}