{"product_id":"rat-p-ampk-phosphorylated-adenosine-monophosphate-activated-protein-kinase-elisa-kit-elk0962","title":"Rat p-AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit - ELK0962","description":"\u003cp\u003eRat p-AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0962-48, ELK0962-96, ELK0962-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: p-PRKAA1; p-AMPK; p-AMPKa1; p-AMPK alpha 1\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 1.25 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 3.13-200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Enzyme and Kinase\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211553570995,"sku":"ELK0962-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211553603763,"sku":"ELK0962-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200646832307,"sku":"ELK0962-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_8e18b4ab-95fc-4d04-9d9b-a008789feb4c.png?v=1767060916","url":"https:\/\/afsbio.com\/products\/rat-p-ampk-phosphorylated-adenosine-monophosphate-activated-protein-kinase-elisa-kit-elk0962","provider":"AFSBio Inc.","version":"1.0","type":"link"}