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Rat PKCe (Protein Kinase C Epsilon) ELISA Kit

Rat PKCe (Protein Kinase C Epsilon) ELISA Kit

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Catalogue Number: ELK4533

Alternative Names: PKC-E; PRKCE; PRKC-E; NPKC-Epsilon

Species: Rat

Assay Type: Sandwich

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research: Signal transduction;Enzyme & Kinase;Apoptosis;Endocrinology;Cardiovascular biology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Epsilon(PKCe). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Kinase C Epsilon(PKCe). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase C Epsilon(PKCe), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase C Epsilon(PKCe) in the samples is then determined by comparing the OD of the samples to the standard curve.