{"product_id":"rat-pnf-h-phosphorylated-neurofilament-h-elisa-kit-elk6916","title":"Rat pNF-H (Phosphorylated Neurofilament H) ELISA Kit - ELK6916","description":"\u003cp\u003eRat pNF-H (Phosphorylated Neurofilament H) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK6916-48, ELK6916-96, ELK6916-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: NFH; 200 kDa neurofilament protein; Neurofilament triplet H protein; NEFH; Neurofilament, Heavy Polypeptide\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 5.5 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 1000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 15.63-1000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Neuroscience\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pNF-H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pNF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pNF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pNF-H in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211966972083,"sku":"ELK6916-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211967004851,"sku":"ELK6916-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200756605107,"sku":"ELK6916-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_dee32220-db77-4f1d-86ad-1c2c7a304075.png?v=1767063309","url":"https:\/\/afsbio.com\/products\/rat-pnf-h-phosphorylated-neurofilament-h-elisa-kit-elk6916","provider":"AFSBio Inc.","version":"1.0","type":"link"}