{"product_id":"rat-sc5b-9-soluble-terminal-complement-complex-c5b-9-elisa-kit-elk10683","title":"Rat sC5b-9 (Soluble Terminal Complement Complex C5b-9) ELISA Kit - ELK10683","description":"\u003cp\u003eRat sC5b-9 (Soluble Terminal Complement Complex C5b-9) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK10683-48, ELK10683-96, ELK10683-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 13.1 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 2000 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 31.25-2000 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction; Metabolic pathway; Apoptosis; Infection immunity;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200670687411,"sku":"ELK10683-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200670720179,"sku":"ELK10683-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200670752947,"sku":"ELK10683-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_8ec34de0-47cb-47b0-8106-f4497be90e25.png?v=1767061363","url":"https:\/\/afsbio.com\/products\/rat-sc5b-9-soluble-terminal-complement-complex-c5b-9-elisa-kit-elk10683","provider":"AFSBio Inc.","version":"1.0","type":"link"}