{"product_id":"rat-tracp-5b-tartrateresistant-acid-phosphatase-5b-elisa-kit-elk9632","title":"Rat TRACP-5b (TartrateResistant Acid Phosphatase 5b) ELISA Kit - ELK9632","description":"\u003cp\u003eRat TRACP-5b (TartrateResistant Acid Phosphatase 5b) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK9632-48, ELK9632-96, ELK9632-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: ACP5; TRAP5b; SPENCDI\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.36 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 40 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.63-40 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Immunology; Cardiovascular\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212835422387,"sku":"ELK9632-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212835455155,"sku":"ELK9632-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200829350067,"sku":"ELK9632-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_c829a684-a1c6-4ca4-bf64-0f565e6ae1da.png?v=1767065085","url":"https:\/\/afsbio.com\/products\/rat-tracp-5b-tartrateresistant-acid-phosphatase-5b-elisa-kit-elk9632","provider":"AFSBio Inc.","version":"1.0","type":"link"}