{"product_id":"rat-tsp-1-thrombospondin-1-elisa-kit-elk10312","title":"Rat TSP-1 (Thrombospondin-1) ELISA Kit - ELK10312","description":"\u003cp\u003eRat TSP-1 (Thrombospondin-1) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK10312-48, ELK10312-96, ELK10312-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.46 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 100 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 1.56-100 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Plasma\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Infection immunity; Hematology;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: Q71SA3\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45199919022259,"sku":"ELK10312-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45199919055027,"sku":"ELK10312-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45199919087795,"sku":"ELK10312-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_93b3c609-d94c-4c46-9389-763fc0b6b40b.png?v=1767028971","url":"https:\/\/afsbio.com\/products\/rat-tsp-1-thrombospondin-1-elisa-kit-elk10312","provider":"AFSBio Inc.","version":"1.0","type":"link"}