{"product_id":"rcc1-monoclonal-antibody-mb66914","title":"RCC1 Monoclonal Antibody-MB66914","description":"\u003cp\u003eRCC1 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66914-50, MB66914-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P18754\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/1000 - 1\/2000)\u003c\/p\u003e\n\n\u003cp\u003eBackground: The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport . Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor activity, which catalyzes the exchange of bound GDP for GTP. Regulator of chromatin condensation 1  is the only known RanGEF . RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally. Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation and methylation. RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis . In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclin B\/cdc2 . This phosphorylation may play a role in RCC1 interaction with chromatin and RCC1 RanGEF activity .\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: This antibody is purified through a protein G column.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of RCC1 protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of RCC1 expression in Hela (A), HL60 (B), COS7 (C), C2C12 (D) whole cell lysates.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 45 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CHC1; Regulator of chromosome condensation; Cell cycle regulatory protein; Chromosome condensation protein 1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human RCC1. The exact sequence is proprietary.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42906393411763,"sku":"MB66914-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42906393444531,"sku":"MB66914-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_4ec4e568-004e-415f-a3d5-68b698cbedd9.jpg?v=1702919008","url":"https:\/\/afsbio.com\/products\/rcc1-monoclonal-antibody-mb66914","provider":"AFSBio Inc.","version":"1.0","type":"link"}