{"product_id":"recombinant-2019-ncov-guanine-n7_meth-n-6his-ept003","title":"Recombinant 2019-nCoV Guanine-N7_meth (N-6His) - EPT003","description":"\u003cp\u003eRecombinant 2019-nCoV Guanine-N7_meth (N-6His)\u003c\/p\u003e\n\n\u003cp\u003eSize: 50ug\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: EPT003-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: SARS-CoV 2 nsp14; SARS-CoV 2 ExoN; Guanine-N7 methyltransferase\u003c\/p\u003e\n\n\u003cp\u003eDescription: Recombinant 2019-nCoV Guanine-N7 methyltransferase is produced by our E.coli expression system and the target gene encoding Ala1-Gln527 is expressed with a 6His tag at the N-terminus.\u003c\/p\u003e\n\n\u003cp\u003eAccession: YP_009725309.1\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 62.9 KDa\u003c\/p\u003e\n\n\u003cp\u003eApparent Molecular Weight: 60 KDa, reducing conditions\u003c\/p\u003e\n\n\u003cp\u003eStorage Condition: Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\n\n\u003cp\u003ePurity: Greater than 85% as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eDelivery Condition: The product is shipped on dry ice\/polar packs. Upon receipt, store it immediately at the temperature listed below.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.\u003c\/p\u003e \n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"Default Title","offer_id":43211010572467,"sku":"EPT003-50","price":1174.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_ee3c1917-d58c-4247-8d0e-2acb2d4e5300.png?v=1767117504","url":"https:\/\/afsbio.com\/products\/recombinant-2019-ncov-guanine-n7_meth-n-6his-ept003","provider":"AFSBio Inc.","version":"1.0","type":"link"}