{"product_id":"recombinant-2019-ncov-nsp7-protein-his-tag-pksr030469","title":"Recombinant 2019-nCoV NSP7 Protein (His Tag) - PKSR030469","description":"\u003cp\u003eRecombinant 2019-nCoV NSP7 Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSR030469-10, PKSR030469-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: NSP7\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: SARS-CoV 2 nsp7\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: SARS-CoV-2\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: E.coli\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eAccession: YP_009725303.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: The ～30kb positive-stranded RNA genome of coronaviruses encodes a replication\/transcription machinery that is unusually complex and composed of 16 nonstructural proteins (nsps). The four proteins nsp7 to nsp10, which are conserved among all CoVs but have no functional homologs outside of the Coronaviridae, are translated as part of the viral polyproteins pp1a and pp1ab, and the mature proteins are released by the action of the SARS-CoV protease nsp5. Hexadecamer of nsp7 and nsp8 may possess dsRNA-binding activity. SARS-CoV 2 nonstructural protein 7 (nsp7) is of interest for its potential roles in the transcription and replication of the positive-stranded viral RNA genome.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Ser1-Gln83\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 8.5.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 12.3 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 12 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43149094453427,"sku":"PKSR030469-10","price":308.0,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43149094486195,"sku":"PKSR030469-50","price":700.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_23987666-4867-40f9-9cb9-d3c42228a18c.png?v=1710340564","url":"https:\/\/afsbio.com\/products\/recombinant-2019-ncov-nsp7-protein-his-tag-pksr030469","provider":"AFSBio Inc.","version":"1.0","type":"link"}