{"product_id":"recombinant-2019-ncov-nucleoprotein-np-protein-his-tag-pksr030506","title":"Recombinant 2019-nCoV Nucleoprotein \/ NP Protein (His Tag) - PKSR030506","description":"\u003cp\u003eRecombinant 2019-nCoV Nucleoprotein \/ NP Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSize: 100μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: PKSR030506-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: Nucleoprotein, NP\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: 2019-nCoV coronavirus NP Protein, 2019-nCoV np Protein, 2019-nCoV novel coronavirus Nucleoprotein Protein\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: SARS-CoV-2\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: Baculovirus-Insect Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-6His\u003c\/p\u003e\n\n\u003cp\u003eAccession: NP_828858.1\u003c\/p\u003e\n\n\u003cp\u003eBackground: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Met1-Ala422\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 80 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: LyopHilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 47.5 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 47.1 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"100μg","offer_id":43155017531571,"sku":"PKSR030506-100","price":1547.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_a32566a4-63bb-4582-8ef3-b519a3390fd3.png?v=1710436516","url":"https:\/\/afsbio.com\/products\/recombinant-2019-ncov-nucleoprotein-np-protein-his-tag-pksr030506","provider":"AFSBio Inc.","version":"1.0","type":"link"}