{"product_id":"recombinant-cntf-human","title":"Recombinant CNTF, Human -","description":"\u003cp\u003eRecombinant CNTF, Human\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: -10, -50\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eSource: Escherichia coli\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: 22.8 kDa, observed by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95% as analyzed by SDS-PAGE and HPLC.\u003c\/p\u003e\n\n\u003cp\u003eBiological Activity: ED50 \u0026lt; 200 ng\/ml, measured cell proliferation assay using TF-1 cells, corresponding to a specific activity of \u0026gt; 5×10ˆ3 units\/mg.\u003c\/p\u003e\n\n\u003cp\u003ePhysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized after extensive dialysis against PBS.\u003c\/p\u003e\n\n\u003cp\u003eAA Sequence: AFT EHSPLTPHRR DLCSRSIWLA RKIRSDLTAL TESYVKHQGL NKNINLDSAD GMPVASTDQW SELTEAERLQ ENLQAYRTFH VLLARLLEDQ \u003cbr\u003e\n QVHFTPTEGD FHQAIHTLLL QVAAFAYQIE ELMILLEYKI PRNEADGMPI NVGDGGLFEK KLWGLKVLQE LSQWt VRSIH DLRFISSHQT GIPARGSHYI \u003cbr\u003e\n ANNKKM\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 0.2 EU\/μg, determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Reconstituted in ddH2O or PBS at 100 μg\/ml.\u003c\/p\u003e\n\n\u003cp\u003eStorage: Lyophilized recombinant human Ciliary Neurotrophic Factor (rhCNTF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhCNTF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.\u003c\/p\u003e\n\n\u003cp\u003eUsage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.\u003c\/p\u003e\n\n\u003cp\u003eDescription: Ciliary Neurotrophic Factor (CNTF) is a cytokine belonging to the Interleukin 6 (IL-6) family, which also includes IL-6, Oncostatin M, Leukemia Inhibitory Factor (LIF), and Cardiotrophin-1. Structurally, CNTF resembles a four-helix bundle composition, similar to the other members of the IL-6 family. The receptor for CNTF is composed of three parts: a gp130-like subunit common in the IL-6 receptor family, a LIF Receptor β subunit, and a CNTF specific α receptor subunit. Upon binding to the CNTF, the β subunit of the CNTF receptor will undergo tyrosine phosphorylation, and activate the intracellular JAK\/STAT pathway. The main function of CNTF in vivo is to promote the differentiation and survival of a variety of neurons and glial cells, including sympathetic precursor cells and spinal motor neurons.Recombinant human Ciliary Neurotrophic Factor (rhCNTF) produced in E. coli is a single non-glycosylated polypeptide chain containing 199 amino acids. A fully biologically active molecule, rhCNTF has a molecular mass of 22.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"10μg","offer_id":42882232418483,"sku":"-10","price":217.41,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":42882232451251,"sku":"-50","price":691.77,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_3e1c7ad5-f79a-48f4-aa41-b1795582adfc.jpg?v=1702049762","url":"https:\/\/afsbio.com\/products\/recombinant-cntf-human","provider":"AFSBio Inc.","version":"1.0","type":"link"}