{"product_id":"recombinant-cynomolgus-5-nucleotidase-cd73-nt5e-protein-his-tag-pksq050049","title":"Recombinant Cynomolgus 5'-Nucleotidase\/CD73\/NT5E Protein (His Tag) - PKSQ050049","description":"\u003cp\u003eRecombinant Cynomolgus 5'-Nucleotidase\/CD73\/NT5E Protein (His Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSQ050049-10, PKSQ050049-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: CD73; NT5E\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: 5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: G7P3J1\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Cynomolgus macaques\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-His\u003c\/p\u003e\n\n\u003cp\u003eAccession: G7P3J1\u003c\/p\u003e\n\n\u003cp\u003eBackground: CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Trp27-Lys547\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Not Applicable\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 58.8 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 58-80 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice\/gel packs. Upon receipt, store it immediately at \u0026lt; - 20°C.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43072227770547,"sku":"PKSQ050049-10","price":625.8,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43072227803315,"sku":"PKSQ050049-50","price":1143.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_3d9b2218-7493-4479-a8b5-db792bb74568.png?v=1708684677","url":"https:\/\/afsbio.com\/products\/recombinant-cynomolgus-5-nucleotidase-cd73-nt5e-protein-his-tag-pksq050049","provider":"AFSBio Inc.","version":"1.0","type":"link"}