{"product_id":"recombinant-cynomolgus-cd3e-cd3ε-protein-fc-tag-pksq050030","title":"Recombinant Cynomolgus CD3e\/CD3ε Protein (Fc Tag) - PKSQ050030","description":"\u003cp\u003eRecombinant Cynomolgus CD3e\/CD3ε Protein (Fc Tag)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 10μg, 50μg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: PKSQ050030-10, PKSQ050030-50\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: CD3e; CD3ε\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: CD3 epsilon; CD3e antigen; CD3e antigen; epsilon polypeptide (TiT3 complex); CD3e molecule; epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex; epsilon subunit of T3; T-cell surface antigen T3\/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: Q95LI5\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Cynomolgus macaques\u003c\/p\u003e\n\n\u003cp\u003eExpression Host: HEK293 Cells\u003c\/p\u003e\n\n\u003cp\u003eFusion Tag: C-Fc\u003c\/p\u003e\n\n\u003cp\u003eAccession: Q95LI5\u003c\/p\u003e\n\n\u003cp\u003eBackground: T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha\/beta and gamma\/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.\u003c\/p\u003e\n\n\u003cp\u003eSequence: Gln22-Asp117\u003c\/p\u003e\n\n\u003cp\u003ePurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM Glycine, pH 7.5.\u003cbr\u003e\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\u003cbr\u003e\nPlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\n\n\u003cp\u003eReconstitution: Please refer to the printed manual for detailed information.\u003c\/p\u003e\n\n\u003cp\u003eEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 38 kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 38-55 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43072226295987,"sku":"PKSQ050030-10","price":180.6,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43072226328755,"sku":"PKSQ050030-50","price":525.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_d04320b2-422f-44d1-856c-2515333fc076.png?v=1708684637","url":"https:\/\/afsbio.com\/products\/recombinant-cynomolgus-cd3e-cd3%ce%b5-protein-fc-tag-pksq050030","provider":"AFSBio Inc.","version":"1.0","type":"link"}