{"product_id":"recombinant-cynomolgus-lag-3-cd223-protein-his-tag-pksq050011","title":"Recombinant Cynomolgus LAG-3\/CD223 Protein (His Tag) - PKSQ050011","description":"Recombinant Cynomolgus LAG-3\/CD223 Protein (His Tag)\n\nSizes: 10μg, 50μg\n\nCatalogue Numbers: PKSQ050011-10, PKSQ050011-50\n\nCitations, Manuals and MSDS Available upon request.\n\nAbbreviation: LAG-3; CD223\n\nTarget Symptom: Lymphocyte Activating 3; LAG3; LAG-3; CD223\n\nTarget Species: Cynomolgus macaques\n\nExpression Host: HEK293 Cells\n\nApplication: ELISA\n\nFusion Tag: N-His\n\nAccession: XP_005570011.1\n\nBackground: Lymphocyte Activating 3(LAG3) belongs to immunoglobulin (Ig) superfamily, Macaca fascicularis LAG3 cDNA encodes 533 amino acids (aa) that include a 17 aa signal sequence, a 451 aa extracellular domain (ECD) with two Ig-like domains, a transmembrane region and a highly charged cytoplasmic region. LAG3' s main ligand is MHC class II, to which it binds with higher affinity than CD4. The protein negatively regulates cellular proliferation, activation, and homeostasis of T cells, in a similar fashion to CTLA-4 and PD-1 and has been reported to play a role in Treg suppressive function.\n\nActivity: Immobilized Cynomolgus LAG-3-His at 2μg\/ml(100 μl\/well) can bind Human FGL1-Fc. The ED50 of Cynomolgus LAG-3-His is 5. 8 ug\/ml.\n\nSequence: Pro23-Leu450(Pro74)\n\nPurity: \u0026gt; 95 % as determined by reducing SDS-PAGE.\n\nFormulation: Lyophilized from a 0.2 μm filtered solution of PBS, PH7.4.\nNormally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.\nPlease refer to the specific buffer information in the printed manual.\n\nReconstitution: Please refer to the printed manual for detailed information.\n\nEndotoxin: \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\n\nCalculated MW: 47.3 kDa\n\nObserved MW: 55-65\u0026amp;140 kDa\n\nStorage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\n\nShipping: This product is provided as lyophilized powder which is shipped with ice packs.\n\nResearch Use Only","brand":"Elabscience","offers":[{"title":"10μg","offer_id":43114021683379,"sku":"PKSQ050011-10","price":299.6,"currency_code":"CAD","in_stock":true},{"title":"50μg","offer_id":43114021716147,"sku":"PKSQ050011-50","price":529.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_b97ac61c-0b85-40fe-98e5-d0cce0b6a3ae.png?v=1709550347","url":"https:\/\/afsbio.com\/products\/recombinant-cynomolgus-lag-3-cd223-protein-his-tag-pksq050011","provider":"AFSBio Inc.","version":"1.0","type":"link"}